You can use multiple audiences in your single activity as per the requirement by using Experience Targeting.

Yes, as per the requirement they can create segments and they can use existing defined segments as well.

You can create a segment and apply on report.If you want to check how many users having more than 1 visit.Refer below segment.  

Please reach out to adobe support.

@Nikester Happy to help you:) 

You need to create a segments for new and return visits.Visit Number equal to 1 is New and Visit Number greater than 1 is Return.Create a segment on visits level. In the report you can check how many interactions coming from new vs return.How they are performing.Create a reportNew visits and Return ...

Thanks @MichaelNotte for the update.

Can you please clarify on below.Are you getting Target basic call on first page load? Checking this only for target integration.We have observed this kind of issue when we created the audiences in adobe analytics and these audiences are only working for return visits in Target.Can you please tell me...

Thanks @aosorio for the update

Are you checking on page URL using "Launch Switch"? It will be helpful if you share the screenshot of your issue.