Hi @emmak109
Yes, running an live AA test on your web domain can potentially impact the audience selection for your AB tests, as both the AA and AB tests are being executed on the same web pages.
The reason for this is that the AA test, being set at a higher priority (999) than the AB tests (priority 0), will take precedence in serving the content to the users. This means that the audience exposed to the AA test will not be available for the AB tests, as they have already been served the content from the AA test.
To address this - Priorities your AB test over AA test (Change the priority), used the right audience for AB test ( exclude the audience who already came across AA test) and monitor impact and do adjustments as per your test objectives.