@TarinehOl Inspecting the screenshots you shared, you are trying to use a Sequential segment condition of "After 09/30/2025" this is not supported in Streaming Segments.
Streaming Segment refers to a single incoming event within a time window of less than 24 hours. To successfully create the Streaming Segment remove "After 09/30/2025" condition. If you really want to exclude any audiences who met this criteria before 09/30/2025, you can create a Batch Segment and add it as an Exclude condition to avoid all audiences to met this criteria in the past. In streaming segments you can use Insegments. You also need to ensure that you use a event window of less then 24 hours or today as detailed in the help documents for Streaming Segmentation