And I found the Adobe Experience Cloud Identity Service is not enable in my adobe. Does this the reason why I cannot see the segment in target? 

After over 12 hours. I still cannot see the segment in Adobe target. 

Dear Abu, Let me wait for few hours. I though it was an immediate change before. Thanks for your kind reply. Leo

Hi, I've successfully published one segment to experience cloud. But this segment cannot be found in target audience. Could anyone please advise the reason?     Thanks Leo